Mass propagation of bamboo became needful that to bamboo considered as poor man timber for rural and traditional people. Vegetative propagation by rhizome cutting, layering, pre-rooted and pre-rhizome, branch cutting, nodal branch chips, offset and culm division are also common in various bamboo. But such methods have various limitations like large scale multiplication, cost of production, microbial infection, difficult in transportation, etc. So, in this paper special attention is given to mass propagation of Dendrocalamus strictus using apical shoot as explants in vitro process.