The study was conducted to assess the influence of meta-Topolin (mT) and Benzyladenineaminopurine (BAP) on in vitro plantlet regeneration of local and exotic banana cultivars. Champa is one of the local Musa variety of Odisha, India. Benzyladenineaminopurine (BAP) is the most commonly used cytokinin in the in vitro propagation tissue culture industry due to its effectiveness and affordability. The development and achievement in the field of modern tissue culture techniques have made it possible to discover various micropropagation protocols by using natural and synthetic phytohormone for the banana on a commercial scale. The effect of meta-Topolin (mT) and Benzyladenineaminopurine (BAP) on micropropagation and adventitious shoot regeneration of Champa variety was compared. During the multiplication stage of Champa variety a synergetic effect was observed when meta-Topolin (mT) supplemented in MS medium. In Multiplication culture longest shoot length (4.23 ± 0.45 cm), mean final fresh weight (7.46±0.37) and maximum no. of shoot/ explant (9.40 ± 0.74) was obtained as compared to other BAP.