Gloriosa superba L. belonging to family Colchicaceae is very unique plant having large number of medicinal values. It is a perennial herb growing from a seed or fleshy rhizome. Present study on qualitative and quantitative analysis of Gloriosa superba L. The qualitative phytochemical studies were carried out in the solvents viz. Methanol, Chloroform and n-Butanol. The Methanol, Chloroform and n-Butanol tuber and leaves extract of Gloriosa superba L. shows the presence of alkaloid, glycosides, terpenoids, tannin, flavonoids, saponins, steroid and phenols but in tuber of Gloriosa superba L. high intensity of phytochemical than that of leaves. also, the quantitative studies were carried out in the same solvent mentioned above, Alkaloid content in tuber of Gloriosa superba L. that was 2.921, 2.546 and 3.045 µg/ml respectively, and total flavonoids in tuber, 0.845, 0.641 and 0.978 µg/ml respectively and also followed by total content of phenols that was 1.284, 0.652 and 1.361µg/ml.