To collect medicinal plants from Kolli Hills and study their Ethnobotanical view. From, the medicinal plants to determine the bacterial and fungal growth arrest assay. In this present study, the bacterial study to use Agar well diffusion method was using to detect the antimicrobial activity and fungal growth inhibition were recorded by Micro-broth dilution methods. In the study maximum inhibition was observed with methanol extracts against Escherichia coli. From the fungal inhibitory highest activity was recorded on Ipomoea pandurata Linn. On Aspergillus Niger when compared to Andrographis echioides Linn. Against Rhizoctonia solani. Medicinal plants have been planted long before a man can leave his feet on earth. The World health organization approximations that about 80% of the people still be subject to upon plant herbal medicines for the treatment of various infections due to easy convenience with minor side effects. The study was proved that the medicinal plants were targeting to cure microbial disease.