Drought stress is the most well-known natural stress, considered a genuine threat to crop production. A better understanding of the mechanisms related to this abiotic stress is crucial in designing experiments to improve crop plants. The present investigation analyzed the drought tolerance in two varieties of Momordica charantia belongs to the family Cucurbitaceae, viz, Momordica charantia var. charantia (Cultivar) and Momordica charantia var. muricata (Wild) in terms of physiological and biochemical characters. The experiment was carried out in the greenhouse, using a completely randomized design, in the control and test groups. The control group was maintained by daily irrigation, while the test group was subjected to drought stress by withholding irrigation for 3, 5, 7, 9 and 11days duration, each treatment with five replicates. Analysis of physiological characters revealed a stability in the amount of chlorophyll content and relative water content (RWC) in the wild variety compared to that of the cultivar. Moreover, the amount of Proline, total phenol, total protein and MDA together with the activity of APOX, Catalase, showed a significant increase in the wild variety. The study noticed the presence of an effective antioxidative system and secondary metabolites which conferred drought stress tolerance in M. charantia var. muricata.