The characteristic phytochemical constituents are steroids, tri-terpenoid, Anthraquinone glycosides, proteins are established in the concentrates of Costus spicatus flower. Established gentle to direct movement and better anthelmintic action after contrasted with ethanolic separate. Oral administration of effect of ECS to diabetic induced rats at dosage of 500 mg/kg body weight resulted during significant decrease of elevated blood glucose and hepatic transaminase enzyme levels, at different treatment period (0th day, 28th day and 45th day) which also showed the structural changes in cytoarchitecture of STZ induced diabetic rats. (SGOT), (SGPT) and (ALP) levels. Confirmed the effect diabetic medicinal plants in restore in pancreas of ECS in anti-hyperglycaemic activity.