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VOL. 6, ISSUE 3 (2021)
Validated RP-HPLC analytical method for simultaneous estimation of imatinib mesylate and anastrazole in pharmaceutical formulation
Authors
Sai Sree M, Shaik Munwar, Ramarao N
Abstract
A simple, precise, accurate, efficient and reproducible, isocratic Reverse Phase- High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the simultaneous estimation of Imatinib mesylate and Anastrazole in pharmaceutical formulation. Imatinib mesylate and Anastrazole were separated using an Phenomenex Luna 3μ C8(2) 100A˚, LC Column 150 x 45, Shimadzu of 2030 LC Prominence i-series with high detection capabilities of PDA detector and the mobile phase contained a mixture of 0.02M sodium dihydrogen phosphate (pH adjusted to 2 with 0.1% orthophosphoric acid), acetonitrile and water (30:55:15,v/v/v). The flow rate was set to 1ml/min with the response detected at 228nm. The retention time of Imatinib mesylate and Anastrazole was found to be 1.88min, 3.139 min. Linearity for imatinib mesylate, in the range of 100-500µg/ml, for anastrazole in the range of 1-5µg/ml with correlation coefficient of 0.9999. The percentage recovery of Imatinib mesylate and anastrazole was found to be 100.27, 99.75 respectively. Validation parameters such as specificity, linearity, precision, accuracy, robustness, and limit of detection (LOD), limit of quantification (LOQ) were evaluated for the method according to the International Conference on Harmonization (ICH) Q2 R1 guidelines.
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Pages:848-853
How to cite this article:
Sai Sree M, Shaik Munwar, Ramarao N "Validated RP-HPLC analytical method for simultaneous estimation of imatinib mesylate and anastrazole in pharmaceutical formulation ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 848-853
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