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International Journal of
Botany Studies
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VOL. 6, ISSUE 3 (2021)
in-vivo propagation of Aristolochia tagala – A rare medicinal plant species of Assam, India
Authors
Bhaskar Sarma, Upen Deka
Abstract
In-vivo propagation was developed for Aristolochia tagala, a rare medicinal plant, using softwood cuttings in different rooting media and different concentrations of Indole-3- butyric acid (IBA). Softwood cuttings were taken from the soft, succulent new spring growth of the plants. Cuttings were disinfected by soaking in 1% w/v solution of bavistin and treated with four different rooting media i.e. the river sand, 1:1 ratio by volume river sand and peat moss, 1:1 ratio by volume river sand and garden soil, and 1:1:1 ratio by volume river sand, garden soil and peat moss and four different concentrations of rooting hormone IBA (500 ppm, 1000 ppm, 3000 ppm, and 5000 ppm). Data recorded were effective IBA concentrations in cuttings, the effect of the soil media, percentage of survival, mean number, and length of adventitious roots per cutting. The result showed remarkable growth differences in the various rooting media and hormone concentrations for the variables evaluated. Cuttings treated in rooting media containing river sand, garden soil, and peat moss (1:1:1 ratio by volume) with 3000 ppm IBA concentration had the best result of all the parameters observed and therefore recommended for invivo propagation of A. tagala.
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Pages:679-683
How to cite this article:
Bhaskar Sarma, Upen Deka "<em>in-vivo</em> propagation of <em>Aristolochia tagala</em> – A rare medicinal plant species of Assam, India ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 679-683
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