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VOL. 6, ISSUE 3 (2021)
The preliminary phytochemical screening, quantification of phenols and flavonoids and antioxidant potential analysis of leaf sample of Ethnomedicinal plant Actinoscirpus grossus var. kysoor (Roxb.) Noltie (Family: Cyperaceae)
Authors
Riddhi Bhati, Krishna Desai, Nainesh R Modi
Abstract
The use of various plant extracts or bioactive constituents is common in conventional medicine. Traditional herbal medicinal practitioners have identified the therapeutic efficacy of several indigenous plants for various diseases. Synthetic and conventional herbal medicine are indeed derived from natural resources. Actinoscirpus grossus var. kysoor (Robx.) Nolite is a herbaceous plant belonging to family Cyperaceae and is used in Ayurveda as medicinal herb for treatment of various diseases. In the present investigation leaf parts of Actinoscirpus grossus var. kysoor (Robx.) Nolite was assessed for the qualitative and quantitative screening of phytocomponents and free radical scavenging capacity. Methanol and chloroform extract was prepared by cold maceration method. Preliminary phytochemical screening showed better results in methanolic leaf extract compared to chloroform as it revealed the presence of Alkaloids, Carbohydrates, Phenols, Flavonoids, Terpenoids, and Steroids while Saponins and Glycosides were absent. Total phenol content and total flavonoid content was checked by detailed quantitative analysis, and the results reported as 172.17 ± 0.33 mg GAE/g of sample and 26.66 ± 4.409 mg QE/g of sample respectively. The results suggest the superabundance of phenolic and flavonoid components in plants. Anti-oxidant activity was carried out using DPPH radical scavenging assay for the methanolic leaf extract. 50% inhibition concentration value of methanolic leaf extract was found to be 144.01 ± 0.33 µg/mL, which was very nearer to the positive control i.e., 133.63 ± 0.41 µg/mL. The results indicated significant antioxidant potential. The plant was proven to be a good source for preparation of drugs from its extracts which can be very beneficial for advancement in medicine, and its further exploration could make it pharmaceutically important plant.
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Pages:767-773
How to cite this article:
Riddhi Bhati, Krishna Desai, Nainesh R Modi "The preliminary phytochemical screening, quantification of phenols and flavonoids and antioxidant potential analysis of leaf sample of Ethnomedicinal plant <em>Actinoscirpus grossus var. kysoor</em> (Roxb.) Noltie (Family: Cyperaceae) ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 767-773
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