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International Journal of
Botany Studies
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VOL. 6, ISSUE 3 (2021)
Phytochemical and haemolytic activity on Stems of Calotropis gigantea Linn
Authors
Bharat V Jain, Pakhale Rohit Rajendra, Md Rageeb Md Usman
Abstract
Since ancient times, people have used medicinal plants to treat varied diseases. Medicinal plants are the important source of drugs, and many of them that are currently available in the pharma ceutical market are obtained from plant sources. The objective of present studies deals with the preliminary phytochemical studies on the aqueous extract of stems of Calotropis gigantea Linn. And phytochemical test for the identification of active constituents. Saponin was identified by foam test. Since the most characteristic properties of saponin is their ability to cause haemolysis, when added to suspension of blood saponin produced changes in erythrocyte membranes, causes haemoglobin to diffuse into surrounding medium. The aqueous extract of Calotropis gigantea Linn. Produce haemolysis in the test tube 11 (i.e 0.90ml of extract or 0.009gm/ml of extract) and it was calculated by using formula given in the procedure. The haemolytic activity was found to be 722 with reference to standard saponin R i.e. 1000 unit. The result of haemolytic activity is shown in Fig.1.
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Pages:788-790
How to cite this article:
Bharat V Jain, Pakhale Rohit Rajendra, Md Rageeb Md Usman "Phytochemical and haemolytic activity on Stems of <em>Calotropis gigantea </em>Linn ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 788-790
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