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VOL. 6, ISSUE 3 (2021)
in vitro evaluation of antibacterial and molecular docking studies of Gardenia gummifera fruit methanol extract
Authors
Vinay Kumar Nm, Riaz Mahmood, Krishna V, Ravishankara B, Ravi Kumar S, Sachin S Nayaka
Abstract
Gardenia gummifera Linn (Family: Rubiaceae) is used in traditional system of medication to treat numerous ailments such as flatulence for cleaning foul ulcers and wounds, anticonvulsants. To validate the traditional therapeutic claim this study has been undertaken to assess the antibacterial activity of the Gardenia gummifera fruit methanol extract (GFME). Antibacterial screening of the GFME was carried out against human pathogenic clinical isolates i.e. Staphylococcus aureus, Streptococcus pneumoniae, Vibrio cholera, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumonia and further Molecular docking study of GCMS identified compounds was done by docking with bacterial enzyme DNA gyrase. The antibacterial screening of GFME against clinical pathogens showed significant bactericidal activity against the strains Staphylococcus aureus (16.67±0.33), Streptococcus pneumonia (12.00±0.58), Vibrio cholera (12.67±0.33), Pseudomonas aeruginosa (19.33±0.67), and Salmonella typhi (11.67±0.33) as compared to the standard drug ciprofloxacin. The molecular docking of quinic acid against the bacterial enzyme DNA gyrase exhibited a good binding affinity of -4.5 kcal/mol, 7 hydrogen bonds, and hydrophobic interaction with 7 amino acid residues so that quinic acid processes good inhibitor as compared to other 4 compounds. Thus the present study strengthened the traditional medicinal claim of the plant Gardenia gummifera Linn possessing the antibacterial drug.
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Pages:321-325
How to cite this article:
Vinay Kumar Nm, Riaz Mahmood, Krishna V, Ravishankara B, Ravi Kumar S, Sachin S Nayaka "<em>in vitro</em> evaluation of antibacterial and molecular docking studies of <em>Gardenia gummifera</em> fruit methanol extract ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 321-325
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