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VOL. 6, ISSUE 3 (2021)
Detection of salicylic acid and jasmonic acid from extracts using HPLC and HPTLC
Authors
Z Mary Swaroopa, R Jaya Madhuri
Abstract
Plant immunity mainly depends on two plant antagonistic hormones, jasmonic acid JA and salicyic acid SA. Both these hormones control defense responses to different types of microbes. When ever a plant is infected by a pathogen naturally, it triggers SAR by elevating the levels of SA. Plant growth promoting rhizobacteria (PGPR) can suppress the disease in plant by triggering induced systemic resistance (ISR).ISR requires Jasmonic acid (JA) and Ethylene (ET) for accumulation of defense related enzymes and defense related substances against pathogen. Interestingly, simultaneous activation of both the JA/ethylene-dependent ISR pathway and the SA-dependent SAR pathway results in an enhanced level of disease protection. In this present context emphasis on production of salicylic acid, and induction of jasmonate by Bacillus that promotes plant growth is carried out on groundnut plants. Easy detection of these compounds is made possible by HPLC, and HPTLC techniques is been proved in the present experimental analysis. Detection of salicylic acid extracted from the bacterial culture and Jasmonic acid from bacterially treated leaves, using effortless, cost effective, HPLC and HPTLC respectively confers great advantage over traditional techniques.
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Pages:567-574
How to cite this article:
Z Mary Swaroopa, R Jaya Madhuri "Detection of salicylic acid and jasmonic acid from extracts using HPLC and HPTLC ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 567-574
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