Introduction: The present investigation has carried out to shed light on the Nematicidal activity and preliminary phytochemical screening of C. sappan powder. Materials and methods: Collection and extraction of C.sappan: The Pathimugam powder was collected from the commercial market of Kerala state. The extract was made from the powdered plant material (20g) by using Aqueous, Ethanol and Methanol as solvent with Soxhlet apparatus (500 mL) at 60°C. Results: Egg Hatchability Test: The solvents were selected and tested at different concentrations (10, 20, 30 and 40ppm) were tested the egg hatchability of root knot nematode M. incognita (Table 1). The results show a decrease in egg hatchability as increasing concentration of the extracts. The increase in exposure period and increasing concentration also decrease in egg hatchability. The C.sappan 10 ppm extract has been found to be less toxic when compared to other extracts and the hatchability has been found to be decreasing with increasing concentrations from 10 at 5 ppm to 1 at 25 ppm after 24 hours exposure time. The egg hatchability was observed in the decreasing order 10 ppm > 20 ppm > 30ppm > 40ppm. Significant results were observed at p>0.0001. Larval Mortality Test: The present study, bark extract of C. sappan was tested nematicidal activity of second stage juveniles of M. incognita in the laboratory conditions. The juveniles were exposed for 24, 48 and 72 hours exposure 2 time at different concentrations (5, 10, 15 and 20 ppm) and the percentage of larval mortality was increasing with increasing toxicity of the bark extracts. Toxicity of each extracts LC50 and LC90, slope value, chi square value and spontaneous response were calculated (Table 3). Qualitative Analysis of Phytochemicals from C. sappan: Alkaloids, terpenoid, tannin, flavonoids, Steroids and phenolics were present in Ethanolic extract; Alkaloids, terpenoid, tannin and phenols were found in Aqueous extract; all the chemicals except saponins were present in methanolic extract only (Table 4). All the three solvent extracts have shown the negative result of saponin and reducing sugar was absent both in aqueous and ethanol. The solvency of C.sappan, methanol exhibit high when compare with two other solvents. FT-IR Results: Aromatic, aliphatic, ring compounds were present in the experimental samples. The peak values are obtained from FT-IR results shows that 418.55 to 549.71 821.68– Aliphatic Iodo groups, 1035. 77 and 1112.93; 1161.15 – Aliphatic bromo compounds, 1597.06 - Alcohol hydroxyl compound rings, 1737.86 - Hydroxy groups, 2040.69 – dimethyl groups, 2063.83 – Saturated aliphatic groups like methylen C-H bends, 2113.98 – aromatic ring stretch, 2310.72 – Organic nitrates, 2372.44-Methyl groups; 2918.3, 3336.85- Ammonia groups were found in the present experimental samples. Conclusion: The present work identify the functional groups these groups do the wonderful role against the pathogens. An attempt has been made in this work to study the functional derivatives of the sample by observing the position and relative intensities of the band in FTIR.