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VOL. 6, ISSUE 4 (2021)
Indirect organogenesis and influence of benzyl adenine on shoot regeneration from Thevetia peruviana leaf explants
Authors
E A Nesy, Lizzy Mathew
Abstract
Since the seed propagation methods are very slow, an in vitro protocol via indirect organogenesis has been developed using leaf explants of Thevetia peruviana, a potential medicinal plant having high therapeutic value. Effect of Benzyl Adenine (BA) was evaluated individually (1.0 - 5.0 mg/L-1) and in combination with varying concentrations of auxins 2, 4-D (0.5 - 3.0 mg/L-1) and IBA (0.5 - 2.0 mg/L-1) for shoot multiplication. Explants responded very quickly with culture medium, morphologically callus appeared creamy white granular (2, 4-D + BA) and creamy light green meristematic (IBA + BA), but the callus were highly meristematic in BA with numerous shoot initials scattered over the entire surface of whole callus mass. Cytokinin BA (1.0 mg/L-1) alone produced maximum number of microshoots with an average of 22.167 ± 1.518 per leaf explant, instead, in combination with IBA best response (6.444 ± 0.702) was achieved in IBA (0.5 mg/L-1) + BA (1.0 mg/L-1) concentration. All green meristemoid calli from other combinations were transferred into the regeneration medium (IBA - 0.5 mg/L-1 + BA 1.0 mg/L-1) before a final change to BA (1.0 mg/L-1) for further maturation. Detached microshoots were rooted in full strength MS medium fortified with 1.0 mg/L-1 IBA. Around 72 % of the rooted plantlets were acclimatized, so this can be utilised as an effective rapid method to conserve this indigenous medicinal plant for large scale clonal propagation.
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Pages:239-243
How to cite this article:
E A Nesy, Lizzy Mathew "Indirect organogenesis and influence of benzyl adenine on shoot regeneration from<em> Thevetia </em><em>peruviana </em>leaf explants ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 239-243
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