In the present study, a direct approach to fabricating silver nanoparticles (AgNPs) via the leaf extract of Aristolochia tagala has been demonstrated. The synthesized Ag-NPs were characterized by UV-visible, X-ray diffraction (XRD), Fourier-transform infrared (FT-IR), Scanning electron microscopy (SEM), Energy dispersive x-ray (EDX), DLS and ZETA Potential. Formation of silver nanoparticles was authenticated by the disappearance of colour from pale yellow to dark brown in colour after incubation. These results confirmed that the appearance of AgNPs was analyzed by UV- visible spectrum around the peak 420 nm. XRD (X-ray diffractometer) demonstrated the formation of crystalline AgNPs with FCC structure having an average crystalline size of 23 nm from XRD profile. The average grain size of the synthesized silver nanoparticles was about 8mm. FT-IR analysis revealed the presence of different functional groups in the synthesized silver nanoparticles. Antimicrobial activity of the synthesized silver nanoparticles was evaluated against Gram positive and Gram negative bacteria such as Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and fungus Candida albicans. Both the leaf extract of Aristolochia tagala and synthesized silver nanoparticles from the leaves of Aristolochia tagala showed moderate antimicrobial activity. In addition to antimicrobial activity, these synthesized silver nanoparticles were studied for the potential role in photocatalytic performance of effective degradation of MO dye under visible light irradiation conditions.