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VOL. 6, ISSUE 4 (2021)
Development and validation of a forced degradation UPLC method for the simultaneous determination of Trifluoperazine HCl and Trihexyphenidyl HCL in bulk and pharmaceutical dosage form
Authors
S Sangeetha, S Alexandar, B Jaykar
Abstract
An excellent method with simple, precise was developed for Trifluoperazine HCl and Trihexyphenidyl HCl by using Forced degradation UPLC method. The column used was C-18 BEH _ 1.7 µm x 2.1 x 50 mm in ambient temperature. Flow rate was 0.8 ml/min, wavelength of 210 nm, mobile phase used was acetonitrile: water (60:40), Run time 4 min. The percentage purity and RT of Trifluoperazine HCl and Trihexyphenidyl HCl were found to be 99.86 & 99.90% and 1.4 &1.8 min respectively. The validation parameters was carried out, Linearity of Trihexyphenidyl HCl and Trifluoperazine HCl were found to be (5μg/ml to 40μg/ml) (2μg/ml to 16μg/ml) with the correlation coefficient of 0.998 and 0.995 respectively. Intermediate precision, Robustness, LOD LOQ was within the limit as per ICH guidelines. In accuracy the percentage recovery of Trihexyphenidyl HCl and Trifluoperazine HCl in bulk drugs samples was 99.45 to 99.97% and 99.45 to 99.86%respectively which indicates that the method was accurate. Forced Degradation was carried out in three conditions acidic, basic and peroxide condition, degradation takes at basic and peroxide. As per literature review there is no method developed for Trihexyphenidyl HCl and Trifluoperazine HCl in Forced degradation UPLC method.
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Pages:914-921
How to cite this article:
S Sangeetha, S Alexandar, B Jaykar "Development and validation of a forced degradation UPLC method for the simultaneous determination of Trifluoperazine HCl and Trihexyphenidyl HCL in bulk and pharmaceutical dosage form ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 914-921
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