The biocatalysts enzymes are used in multifarious ways in different field. The applications into four broad categories: (i) Therapeutic uses, (ii) Analytical uses, (iii) Manipulative uses, and (iv) Industrial uses. The production of enzymes would be a challenging task hence mass production of enzymes from agricultural wastes would be a emerging trend now. In this present study Agricultural waste Banana stem were used as a substrate from which amylolytic fungal isolate Aspergillus ustus Asp-2910 was subjected to submerged and solid-state fermentation of Amylolytic α-amylase enzyme. Amount of α-amylase liberated by identified mould was quantified by estimating the amount of reducing sugars produced when specified quantity of starch was hydrolyzed after incubation at 40°C with known concentration of enzyme solution. The specific activity was recorded as 4.731 IU/mg for amylase enzyme produced by Aspergillus ustus Asp-2910 in Solid State fermentation and submerged fermentation the activity recorded as 52 IU/mg this results showed the better production of amylolytic enzyme facilitated by Banana stem through submerged cultivation method than solid state method.