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VOL. 6, ISSUE 4 (2021)
Effect of Calotropis procera leaf extracts and partitioned fractions on anti-inflammatory and analgesic activity
Authors
Pushpa Karale, Shashikant Dhawale, Mahesh Karale, Tukaram Kadam
Abstract
The present study was aimed to evaluate the anti-inflammatory and analgesic activities of Calotropis procera (named as CP) using various in-vivo and in-vitro models. Chloroform (CECP), ethanol (EECP) and methanolic (MECP) leaf extracts were screened for anti-inflammatory activity using carrageenan-induced rat paw swelling test and analgesic activity was performed for its central and peripheral pharmacological action by using acetic acid-induced writhing model. In-vitro anti-inflammatory activity of acetone (ACF) and ethyl acetate (EAF) fractions of methanolic extract of C. procera was assessed by using egg albumin denaturation and heat induced hemolysis models. Aspirin was used as a standard drug to evaluate anti-inflammatory and analgesic activities of in-vivo and in-vitro models. MECP significantly (P<0.01) decreased paw edema compared to control group animals. MECP (36.75% inhibition) revealed highly potent anti-inflammatory potential after 3 h, which was even greater than that of aspirin (32.45% inhibition). Similarly, it also exhibited highly significant and more analgesic activity (74.48% reduction) that of aspirin (61.12% reduction). In consistent with the in- vivo anti-inflammatory effects fractions, ACF and EAF also demonstrated its potential inflammatory activity in a concentration-dependant manner in in -vitro models. At 400 μg/mL concentration EAF showed greater % stabilization (46.05%) than aspirin (43.62%) in human red blood cell (HRBC) membrane stabilization assay. The findings of the present study revealed that the MECP possesses potent anti-inflammatory and analgesic activity against all the tested models. Whereas, EAF demonstrated significant in-vitro anti-inflammatory activity.
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Pages:862-867
How to cite this article:
Pushpa Karale, Shashikant Dhawale, Mahesh Karale, Tukaram Kadam "Effect of<em> Calotropis procera </em>leaf extracts and partitioned fractions on anti-inflammatory and analgesic activity ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 862-867
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