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VOL. 6, ISSUE 4 (2021)
β-Lactamase producing bacterial pathogens of clinical samples detection and characterization through molecular techniques
Authors
Dasari Venkateswarlu, Arjun Pandian, Samiraj Ramesh
Abstract
To establish the predominance and genotypes of unlimited variety β-lactamases (ESBLs) amongst hospital clinical isolates of Escherichia coli and Klebsiella pneumoniae, we performed antibiotic susceptibility testing, DNA sequencing analysis. Hence, simple methods were followed herein to isolate and characterize antibiotic resistant bacteria through widespread phenotypic, morphological, biochemical and molecular characters. Among the 520 isolates collected from Prakasam (DT), Costal Andhra, Andhra Pradesh, India. Gene sequence results in E. coli showed no of matches 478, in NCBI blast shows 99.31% similar to bla ndm 5 type of resistance, so below results shows genotypic conformation with ndm primer and classification of resistance type is ndm 5. bla ndm amplicon sequence of K. pneumoniae, based on above analysis ndm primer amplicon shows 99% similarity with bla-ndm 5 type. We confirmed that K. pneumoniae shows 99.09% similarity with bla ndm 5 type of resistance. K. pneumoniae showed no of matches 570, concluded that based on different experimental studies metallo β-lactamses resistance was identified from locally isolated E.coli and K. pneumoniae in provience of Andhra Pradesh, India.
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Pages:901-906
How to cite this article:
Dasari Venkateswarlu, Arjun Pandian, Samiraj Ramesh "β-Lactamase producing bacterial pathogens of clinical samples detection and characterization through molecular techniques ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 901-906
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