Classical Swine Fever (CSF) is a disease caused by a Classical Swine Fever Virus (CSFV). The disease is highly contagious and affects both the domestic pigs and wild boars. The disease poses worldwide problem with large economic loses. The envelope protein E2 is highly immunogenic and therefore represents an important target. Expression of a wide range of foreign proteins in plant systems have gained interest in the recent years. The aim of the present study was to transfer the E2 gene into rice calli via biolistic bombardment method and to investigate the expression of E2 gene of classical swine fever virus in the Transformed rice plants. Confirmation for the presence of E2 gene was done by PCR and Southern bloting. The relative gene expression of the plant-produced E2 was assayed by Real Time PCR. Expression of recombinant E2 protein was immune genically detected by ELISA and Western blotting. A protein band of approximately 60k Da detected by western blot showed good antigenicity of target E2 protein.