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VOL. 6, ISSUE 4 (2021)
Phylogenetic analysis of Evolvulus species based on RBCL secondary structure
Authors
Pavithra M K S, Kannan K P
Abstract
Context: The plant Evolvulus alsinoides (Linn.) Linn (Convolvulaceae), is the source of an important traditional Ayurvedic medicine. Misidentification and adulteration of the plant affects the safety and efficacy of medication. Phylogenetic relationships among the species of this family are unknown. Objective: The present study sought to detect the phylogenetic relationships based on Ribulose-1, 5-bisphosphate carboxylase (RBCL) region of all 99 species of Convolvulaceae recorded. Materials and methods: Accession of six species of Evolvulus served as reference samples. DNA of Evolvulus alsinoides (Linn.) Linn was isolated and the RBCL region was amplified using polymerase chain reaction (PCR). The RBCL sequence of the sample was deposited in the Genbank database and was compared with the RBCL sequences deposited in the NCBI server. Distance analysis, species discrimination and secondary structure of RBCL were used to assess the ability of RBCL sequence in authenticating. The phylogenetic relationships were detected using three methods: Bayesian inference (BI), maximum likelihood (ML), and neighbor joining (NJ). Results: Six species of Evolvulus alsinoides (Linn.) Linn were well resolved in molecular phylogenetic tree. Besides, Evolvulus nuttallianus and Evolvulus sericeus was closer to Evolvulus alsinoides (Linn.) Linn within the tree. Further, we also found that RBCL secondary structurecan be a candidate tool in distinguishing three closely related species Evolvulus nuttallianus, Evolvulus sericeus and Evolvulus alsinoides (Linn.) Linn. For accurate identification of different species of Evolvulus based on species-specific nucleotide sites, a consensus sequences database with all 7 species is established. Discussions and conclusions: The results are able to discriminate Evolvulus species and illustrate the phylogenetic relationships, which are essential for the investigation of adulterants and misidentifications of Evolvulus.
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Pages:952-957
How to cite this article:
Pavithra M K S, Kannan K P "Phylogenetic analysis of <em>Evolvulus</em> species based on RBCL secondary structure ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 952-957
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