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VOL. 6, ISSUE 4 (2021)
Anticancerous efficacy of Mansoa alliacea leaf extracts on human epidermoid carcinoma A431 cells
Authors
Rajanna L, N Vinod
Abstract
Mansoa alliacea (Lam.) A. H. Gentry is a woody vine belonging to Bignoniaceae. The present study evaluated the antincancerous efficacy of M. alliacea leaf extracts against Human epidermoid carcinoma cell line A-431. Leaf extracts were prepared using different solvents viz., petroleum ether, chloroform, ethyl acetate, ethanol and water. Cell viability was tested with MTT assay. Ethyl acetate and ethanol extracts showed better IC 50 values i.e., 71.31µg/ml and 73.32 µg/ml. Further Annexin V and PI staining was used to check apoptosis and CASPASE-3 expression was evaluated using FITC Caspase 3 antibody with camptothecin as positive control. Ethyl acetate and ethanol extract showed 90.42% and 79.82% of early apoptotic cells compared to Standard drug Camptothecin showing 74.53% of early apoptotic cells. These results suggest that the Ethyl acetate and ethanol leaf extracts of M. alliacea may have possible therapeutic potential against Human epidermoid carcinoma cell line A-431.
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Pages:958-964
How to cite this article:
Rajanna L, N Vinod "Anticancerous efficacy of <em>Mansoa alliacea </em>leaf extracts on human epidermoid carcinoma A431 cells ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 958-964
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