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VOL. 6, ISSUE 4 (2021)
In vitro antioxidant investigations on Baptisia tinctoria roots
Authors
Suraj Banal, Rakesh Chawla, Vijender Kumar, Sanjeev Mittal
Abstract
Traditionally, roots of Baptisia tinctoria (Wild indigo, Indigo weed; Fabaceae) have been utilized in the Indian frameworks of medication for the treatment of various ailments. The current examination was designed to evaluate antioxidant properties of B. tinctoria roots. The antioxidant action was resolved utilizing 1, 1-diphenyl-2-picrylhyrazyl (DPPH) radical strategy. The various extracts of B. tinctoria roots were prepared in the increasing order of polarity such as petroleum ether (60-80ºC), chloroform, methanol and water using standard protocols. As polyphenols are the significant class of phytoconstituents present in chosen plant, in this manner it was intended to isolate polyphenolic rich parts from methanol concentrate. The ethyl acetate fraction (IC50 = 37.70 µg/ml) of methanol extract of plant roots exhibited maximum antioxidant action followed by methanol extract (IC50 = 80.76 µg/ml) and chloroform extract (IC50 = 197.36 µg/ml) in comparison to ascorbic acid (IC50 = 19.96 µg/ml). On the other side petroleum ether extract (IC50 = 475.27 µg/ml) and water extract (IC50 = 386.26 µg/ml) did not show any inhibitory impact on stable DPPH radicals. Phytochemical screening of plant roots showed presence of polyphenols. Polyphenols have been considered as common antioxidant agents on the basis of literature data. At last, it is suggested that phenols and flavonoids are answerable for antioxidant action of chosen plant. Further, the column chromatography studies will be planned to isolate these phenols and flavonoids compounds responsible for antioxidant activity.
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Pages:296-300
How to cite this article:
Suraj Banal, Rakesh Chawla, Vijender Kumar, Sanjeev Mittal "<em>In vitro</em> antioxidant investigations on <em>Baptisia tinctoria</em> roots ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 296-300
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