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VOL. 6, ISSUE 4 (2021)
Caesalpinia crista coat protects platelets and plasma cells from sodium nitrite induced oxidative stress and exhibits anticoagulation property
Authors
Chandramma Srinivasa, Jayanna Kengaiah, Sharath Kumar M Nandish, Chethana Ramachandraiah, Ashwini Shivaiah, Devaraja Sannaningaiah
Abstract
Current study investigates the protective effect of Caesalpinia Crista Coat Methanolic Extract (CCCME) on NaNo2 (10mM)-induced oxidative stress on plasma and platelets along with its anticoagulation potential. As oxidative stress markers, the level of lipid peroxidization (LPO), Protein carbonyl content (PCC) endogenous antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) activities were measured. The level of LPO and PCC in sodium nitrite induced oxidative stress in platelets and plasma cells increased. While, the activity of SOD and CAT decreased. Interestingly, CCCME significantly normalized the increased level of LPO, PCC level in a dose dependent manner from the concentration 0-150μg. On the other hand, CCCME normalized the activity of SOD and CAT in sodium nitrite induced oxidative stress in platelets and plasma cells significantly and the maximum dose consumed was 200µg. The CCCME showed strong anticoagulant effect as enhanced the clotting time of both PRP and PPP from control 199s to 680s and 250s to 753s respectively. The anticlotting effect of CCCME was also further strengthened by APTT and PT test. CCCME appeared to interfere in intrinsic pathway of coagulation. CCCME could be better candidate in the regulation of stress related complications.
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Pages:362-368
How to cite this article:
Chandramma Srinivasa, Jayanna Kengaiah, Sharath Kumar M Nandish, Chethana Ramachandraiah, Ashwini Shivaiah, Devaraja Sannaningaiah "<em>Caesalpinia crista</em> coat protects platelets and plasma cells from sodium nitrite induced oxidative stress and exhibits anticoagulation property ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 362-368
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