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VOL. 6, ISSUE 4 (2021)
Pharmacognostical and phytochemical studies on roots of Smilax perfoliata– Alternate source for the ayurvedic drug chopachinee
Authors
R Gowri, Manoj Kumar D
Abstract
Introduction: Chopachinee is one of the important Ayurvedic drug used in the treatment of several diseases. The accepted botanical source of Chopachini is Smilax china. Smilax perfoliata is used as a substitute for the Ayurvedic drug Chopachini. S. perfoliata is used traditionally in the treatment of epilepsy, psychosis, diseases of nervous system and Parkinsonism. The aim and objectives of the present study is to evaluate the pharmacognostical and phytochemical studies on the roots of S. perfoliata Methods: Pharmacognostical studies comprises of taxonomical, macroscopical, microscopical studies including powder and macerate analysis. The phytochemical study involves determination of physicochemical constants, fluorescence analysis, histochemical tests, preliminary phytochemical screening and HPTLC studies. Quantification of secondary metabolites like total phenolic, flavonoid and alkaloid content was carried out. Quantity of Diosgenin present in the extract was determined by HPLC method. Results: Transectional view of the roots showed outer periderm and inner ground parenchyma with diffusely distributed vascular strands. The periderm seems to have originated deep in the vascular strands located inner portion of the rhizome. Macerate of root showed group of parenchyma cells, fibre, vessels with scalariform and reticulate thickenings. Powder revealed the presence of cork cells, fibre, starch grains and vessels. Preliminary phytochemical screening of different extracts revealed the presence various phytoconstituents. The phenolic, flavonoid and alkaloid content of alcohol extract was found to be 0.059 mg GAE/g, 0.2639 mg Q/Eg and 0.526 % w/w respectively. The amount of Diosgenin present in root extract was found to be 0.151 %. Conclusion: The study will help in laying down pharmacopoeial standard for the drug Smilax perfoliata.
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Pages:421-429
How to cite this article:
R Gowri, Manoj Kumar D "Pharmacognostical and phytochemical studies on roots of <em>Smilax perfoliata</em>– Alternate source for the ayurvedic drug chopachinee ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 421-429
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