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VOL. 6, ISSUE 4 (2021)
Identification of phytochemicals and anticancer activity of ethanolic extract from the tubers of Typhonium flagelliforme (Lodd.)
Authors
D Swain, G Pushpalatha, M J Bordoli, P Das
Abstract
Typhonium flagelliforme, is a natural source of anticancer compounds. The present study was an attempt to obtain bioactive fraction of its tuber for the phytochemical characterization and experimental evaluation for anticancer activity. Anti-proliferative activity of the extract was studied on cancer cell lines of different tissue origin i.e., MCF-7, L6, PA1, MIApaCa2 and A549. The tuber extract was found to bind tubulin with a reduction in fluorescence intensity in concentration dependent pattern. Owing to interference with tubulin, an induction in apoptotic cell death of MCF-7 cancer cell line was observed. Upon the extract treatment, there was a 15 and 30 % of cell early and late apoptosis respectively, was recorded. A consistent result was demonstrated with the appearance of numerous fragmented nuclei by using DAPI staining. The IC50 value of the extract was found to be 650.8 µg/ml for L6, 677.1 µg/ml for PA1, 704.0 µg/ml for MIApaCa2, 2098.8 µg/ml for A549 and 150.1 µg/ml for MCF-7 cell lines, respectively which reflects modest anti-proliferative activity. Thus, it was concluded that the tuber extract of T. flagelliforme inhibit proliferation of cancer cells of different tissue origin albeit at higher concentration, possessing a great potential as a lead molecule for the management of human cancers.
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Pages:765-771
How to cite this article:
D Swain, G Pushpalatha, M J Bordoli, P Das "Identification of phytochemicals and anticancer activity of ethanolic extract from the tubers of <em>Typhonium flagelliforme </em>(Lodd.) ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 765-771
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