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VOL. 6, ISSUE 4 (2021)
In vitro plant production through stem derived callus of senna (Cassia angustifolia)–a potential source of sennoside
Authors
Pratima Rani Sardar
Abstract
An effective protocol was established for micropropagation of senna using callus developed from the stem. Senna is grown in different parts of India and is extensively used for its medicinal value. The nodal explants were taken from the twigs of the plant which were growing in the field. It was observed that when inoculated on MS + 5 µM N6 -benzyladenine (BA) medium induced brownish black basal callus. Such calluses on transfer to Murashige and Skoog’s medium containing BA or 2iP or Kn differentiated shoot buds within 7 to 10 days which organised shoots in next 10 days. Among these cytokinins, the best response was achieved on MS medium containing 5 µM BA, 100% cultures showed response (5.39±0.3 4 shoots per culture). Nearly 70 % excised shoots organised roots (3.25±0.45 roots per culture) on ½ strength Murashige and Skoog’s medium containing 10µM NAA. Plantlets were gradually hardened in soilrite and acclimatised to soil. The protocol shall be useful for mass propagation as well as improvement of this crop.
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Pages:640-642
How to cite this article:
Pratima Rani Sardar "<em>In vitro</em> plant production through stem derived callus of senna (<em>Cassia</em> <em>angustifolia</em>)–a potential source of sennoside ". International Journal of Botany Studies, Vol 6, Issue 4, 2021, Pages 640-642
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