Medicinal plants have bioactive secondary component, which are used to control of several diseases. Medicinal plant Azima tetracantha which possess own pharmaceutical activities and are applied directly to snake bite, stomach disorders, toothache, bleeding gums, rheumatism and chronic diarrhea. In the present investigation the phytochemical studies were carried out in Azima tetracantha leaves and to detect the presence of secondary metabolites like alkaloids, steroids, flavonoids, terpenoids, saponins, tannins, phenols, coumarins and glycosides. The solvent system selected for the results of TLC was 100 ml petroleum ether, 11ml acetone and 1ml distilled water and identified spot in the aqueous extract. The Rf values of the extract was 0.2. The study will provide the presence of secondary metabolites for the leaf extract of Azima tetracantha to exhibit therapeutic properties.