ARCHIVES
VOL. 6, ISSUE 5 (2021)
A comparison of Bradford, Lowry and UV-280 methods to determine the protein content from green and red Christia vespertilionis leaves
Authors
Izzah Farhah Zambari, Nur Airina Muhamad, Sitti Rahma Abdul Hafid
Abstract
Christia vespertilionis comprises two types, namely green and red leaves. Both have biological compounds that promote anti-cancer and anti-inflammatory activities. Protein content has been used as a parameter to analyse the potentials of C. vespertilionis as a medicinal plant. Thus, this study is aimed at comparing three spectrophotometric methods (Bradford, Lowry and UV-280) to determine the protein content of C. vespertilionis leaves in a precise and accurate way. The protein content in the purified extract of green supernatant (GS), green pellet (GP), red supernatant (RS) and red pellet (RP) of C. vespertilionis leaves was determined by Bradford’s, Lowry’s and UV-280 methods, respectively. GP contained the highest protein content for all the methods with Bradford as the highest sensitivity at 4.51±0.040 mg/ml. Whilst, Lowry in the moderate sensitivity, at 0.528 ± 0.005 mg/ml and UV-280 in the lowest sensitivity with 0.293 ± 0.003 mg/ml protein content. Based on the results, the Bradford method shows more reliable protein values than Lowry and UV-280, due to high sensitivity and precision. This study is of much significance as it helps in acknowledging the most accurate and precise method for determining protein content.
Download
Pages:508-510
How to cite this article:
Izzah Farhah Zambari, Nur Airina Muhamad, Sitti Rahma Abdul Hafid "A comparison of Bradford, Lowry and UV-280 methods to determine the protein content from green and red <em>Christia vespertilionis</em> leaves ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 508-510
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.