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VOL. 6, ISSUE 5 (2021)
Agar well diffusion: A prominent method for in vitro screening of antimicrobials
Authors
Juhee Ansari, Shom Prakash Kushwaha, Vaseem Ahmad Ansari, Kuldeep Singh, Syed Misbahul Hasan
Abstract
Antimicrobial fend off millions of casualties every year. Unsuitable prescription and overdoing of antimicrobial have steered resistance as a global health emergency and results in 700,000 mortalities a year. The World Health Organization cogitates antimicrobial resistance as a universal threat to human health. Novel resistance mechanisms are evolving and dispersing globally, bullying our capability to treat infectious diseases. The increasing difficulty in treating bacterial and fungal infections is due to the perpetual issue of microbial resistance to antibiotics. The primary goal of antimicrobial research insists on avoiding resistance by overcoming existing bacterial cell processes and reducing side effects. Agar well diffusion method involves a simple and efficient experimental procedure. To infiltrate the agar plate, it is smeared with a dissemination of the microbial inoculum across the whole surface. Following this, a well is aseptically poked with a sterile cork borer and the test/standard solution is put into the well. Next, agar plates are cultured in context to the kind of microorganism being tested. The antimicrobial ingredient permeates the agar medium and interferes with the development of the tested microbial strain.
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Pages:836-839
How to cite this article:
Juhee Ansari, Shom Prakash Kushwaha, Vaseem Ahmad Ansari, Kuldeep Singh, Syed Misbahul Hasan "Agar well diffusion: A prominent method for <em>in vitro</em> screening of antimicrobials ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 836-839
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