Tomato (Solanum lycopersicum L.) is a most popular and widely grown vegetable crop in the world. Tomato (Solanum lycopersicum L.) is one of the most important vegetable crops under solanaceous group which can be grown both under open field conditions and greenhouse. Gamma rays induced early flowering (sample 1) and late flowering (sample 2) mutants pollens were used for the present study. SEM-EDS analyses were studied in the obtained tomato pollen grains. Scanning electron microscope (SEM) and Fourier transform infrared (FTIR) were employed to observe possible variation in the microstructures and spectral characteristics respectively. The FTIR spectral of control and processed samples showed slight variations in peak positions. The SEM images of the sample 1 and sample 2 tomatoes pollen grains showed some level of micro structural dissimilarities compared with control.