The present study was undertaken to study the effect triacontanol (TRIA) on the growth, antioxidant enzyme activities and glutathione metabolism in radish (Raphanus sativus L.) seedlings subjected to cadmium (Cd) stress. Cd at 1mM conc decrease thiol content and total glutathione levels, a clear indication of oxidative stress. TRIA supplemented to Cd (1mM) treated seeds effectively restored total glutathione levels and maintained cellular redox status in equilibrium. Cd (1mM) induced lipid peroxidation and increased H2O2 level but inhibited glutathione reductase (GR, E.C. 1.6.4.2) enzyme activity. Further, supplementation of TRIA restored and enhanced the functional activity of ascorbate peroxidase (APX, E.C. 1.11.1.11), γ-Glutamylcysteine synthetase (γ-GCS, E.C. 6.3.2.2) and glutathione-S-transferase (GST, E.C. 2.5.1.18) activity to a greater extent then Cd-alone treatment. These results clearly indicate the protective role of TRIA in modulating the redox status of the plant system through the antioxidant pathway for combating Cd-induced oxidative stress.