The present study deals with the systematic account of 28 taxa of family Scenedesmaceae, order Sphaeropleales, (formerly belonging to the order Chlorococcales), collected from Periyar River in Kerala. They include the genera, namely, Acutodesmus (1), Desmodesmus (7), Scenedesmus (7), Tetradesmus (4), Westella (1), Coelastrum (5) Hariotina (1), Asterarcys (1) and Dimorphococcus (1). Out of these, three taxa are new to Kerala and most of them are new records from Periyar River.